phosphorylated p‑ smad1 5  (Cell Signaling Technology Inc)

Journal: Oncology Reports

Article Title: Anti-proliferative effect of honokiol on SW620 cells through upregulating BMP7 expression via the TGF-β1/p53 signaling pathway

doi: 10.3892/or.2020.7745

Figure Lengend Snippet: Effects of HNK on TGF-β1 and p53 in SW620 cells. (A) The effect of HNK on the protein levels of Smad1/5/9 and p-Smad1/5/9 in SW620 cells was evaluated by western blot and quantitative analysis (GAPDH was used as a loading control). (B) Western blot and (C) quantitative analyses were used to assess the effect of HNK on TGF-β1 expression in SW620 cells. The effect of HNK on p53 and p-p53 levels was determined by using (D) western blot assays and (E) quantitative analyses; the ratio of p-p53/total p53 was determined by quantitative analyses. The control was treated with 6 µl DMSO (equivalent to HNK 30 µM). *P<0.05, **P<0.01 vs. control groups. HNK, honokiol; TGF, transforming growth factor; p-, phosphorylated.

Article Snippet: SW620 cells were treated with 5 µm LY364947 at 37°C for 24 or 48 h. The primary antibodies used were as follows: GAPDH (cat. no. 10494-1-AP), Bad (cat. no. 10435-1-AP), Bcl-2 (cat. no. 60178-1-Ig), proliferating cell nuclear antigen (PCNA; cat. no. 10205-2-AP), BMP7 (cat. no. 12221-1-AP) and TGF-β1 (cat. no. 21898-1-AP; all from Proteintech Technology, Inc.); Smad1/5/9 (cat. no. sc-6031-R), phosphorylated (p)-Smad1/5/9 (cat. no. sc-12353), Smad2/3 (cat. no. sc-8332) and p-Smad2/3 (cat. no. sc-11769; all from Santa Cruz Biotechnology, Inc.); p53 (cat. no. A11232) and p-p53 (cat. no. AP0083; both from Abclonal Technology, Inc.).

Techniques: Western Blot, Expressing